Nocardia Isolation by Paraffin Baiting Technique

Nocardia Isolation by Paraffin Baiting Technique Nocardia isolation from clinical samples with paraffin baiting technique Abstract Background: The genus Nocardia is cause infection in lung, skin, brain, cerebrospinal, eyes, joints and kidneys. This bacterium is slow-growing and it is difficult to isolate of polymicrobial specimens. Several methods have been reported for Nocardia isolation from clinical samples. In current study, we used of three methods such as paraffin baiting technique, paraffin agar, and conventional media for Nocardia isolation of various clinical specimens from Iranian patients. Methods: In this study, we collected five hundred and seventeen from various clinical specimens including sputum of patients with suspected tuberculosis, bronchoalveolar lavage, sputum of patients withcystic fibrosis, trachea, cutaneous and subcutaneous abscess, cerebrospinal fluid, dental abscess, mycetoma, wound, bone marrow biopsy, and gastric lavage. Smears of all clinical specimens were investigated with Gram stain, partially acid fast and kinyoun stain. All collected specimens were cultured on to carbon free broth tube (paraffin baiting technique), paraffin agar, sabouraud dextrose agar, sabouraud dextrose agar with cycloheximide and incubated at 35 °C. Results: In direct microscopy, partially acid fast and Gram staining were seen positive for five and three clinical specimens respectively and the kinyoun stain were negative for all isolates. Seven isolates of clinical specimens were isolated with paraffin baiting technique. In our study, paraffin baiting technique is more effective than conventional media and paraffin agar for Nocardia isolation. Conclusions: In the present study, shown that use of paraffin baiting technique is more effective of other methods for Nocardia isolation of various clinical specimens. Key words: Nocardia, Paraffin baiting technique, Paraffin agar, Sabouraud dextrose agar Introduction Nocardia spp. are group aerobic actinomycetes, gram positive rods, partially acid fast, non-motile, filamentous branches, catalase positive and methenamine silver-positive [1-3]. The genus Nocardia is opportunistic pathogens [2] that are found around the natural environments. This bacterium is not part of normal microbialflora in human body and animals [1, 4, 5] as well as, there is no report of person to person transmission [5]. This microorganism first introduced by Edmond in 1888 [1, 6]. Nocardia species are cause serious infections in different parts of the body especially lung and skin [6]. Nocardial infections acquired via inhalation of aerosolsor skin damage [7]. In recent years, nocardiosis increased in immune disorder diseases such as Pemphigus disorder, Behà §et’s disease, malignancy, organ transplantation [8-10]. Todate, isolation and identification of Nocardia is improved from clinical specimens [11, 12]. Clinical diagnosis in nocardiosis is controversial and clin ical signs are not specific for this bacterium. Standard tool in Nocardia infections identification are including: isolation and pure culture, Gram stain and partially acid-fast [12, 13]. Nocardia species are slow growing bacteria and isolation this bacterium of polymicrobial specimens is difficult in clinical microbiology laboratory [14]. Decontamination of pulmonary specimens such as sputum is toxic for the genus Nocardia. Paraffin baiting technique was reported for Nocardia and mycobacteria isolation of soil [15]. Paraffin baiting technique was reported that is successfully for Nocardia isolation of various clinical specimens especially poly microbial specimens such as sputum [3,14, 16]. The aim of this study is unique for two reasons: The first aim of this study were comparison of paraffin baiting technique with other methods such as conventional media including sabouraud dextrose agar, sabouraud dextrose agar with cycloheximide and paraffin agar to isolate Nocardia from various clinical specimens such as bronchoalveolar lavage (BAL), sputum of patients with suspected tuberculosis, sputum of patients with cystic fibrosis, cutaneous abscess, cerebrospinal fluid(CSF), dental abscess, mycetoma, trachea, wound, bone marrow biopsy and gastric lavage. The second aim was to estimate the prevalence of Nocardia infection in Iranian patients. There are few numbers of case reports of Nocardia infection and there is no comprehensive database of nocardiosis, therefore, it is essential to better assess the prevalence of this bacteria. Methods Sample collection Five hundred and seventeen various clinical specimens such as sputum of patients with suspected tuberculosis, sputum of patients with cystic fibrosis, BAL, cutaneous and subcutaneous abscess, CSF, dental abscess, mycetoma, wound, bone marrow biopsy, gastric lavage and trachea were collected between February 28, 2011 through March 8, 2013 (Table 1). Direct microscopy All clinical specimens were examined with direct microscopy. The first, clinical specimens were homogenized and were centrifuged in 10000 rpm for 10 minutes and the supernatant was discarded. The smears were prepared from the sediments and were stained with Gram stain, partially acid fast and Kinyoun stain. Culture on different media Sediment of specimens were inoculated on sabouraud dextrose agar (Merck- Germany), sabouraud dextrose agar with cycloheximide(cycloheximide-Sigma-Aldrich-USA), paraffin agar (KH2PO4, K2HPO4, NH4Cl, NH4NO3, MgSO4 .7H20, ZnSO4, FeSO4, MnSO4, Bacto- Agar and Distilled water) and McClungs carbon-free broth tube (MgSO4 7H2O: 0.5 g, ZnSO4: 2 mg, FeCl3: 10 mg, MnCl2. 4H2O: 8 mg, K2HPO4: 0.8 g, NaNO3: 2 g, Distilled water: 1lit, pH 7.2) with paraffin coated glass rod placed. Tubes were incubated at 35 °C for one month with daily controlled [14, 17]. Results Examination of stained smears in direct microscopic, were detected three specimens with Gram staining and five specimens with partially acid fast staining and all smears were negative for Kinyoun stain. In McClungs carbon-free broth, colonies similar cream to white-colored appearing on the paraffin-coated glass rod (Figure 1). Colonies similar to the genus Nocardia were cultured on nutrient agar and were purified (Figure 2). Colonies were stained with Gram positive and partially acid fast and were negative for Kinyoun stain. All clinical isolates were grown in lysozyme broth medium. Seven specimens were positive for the genus Nocardia (1.3%) with paraffin baiting technique as compared with sabouraud dextrose agar and sabouraud dextrose agar with cycloheximide and paraffin agar (Table 1). In comparative to various media, paraffin baiting technique was better in the isolation Nocardia, so this technique is effective and specific for Nocardia isolation of various clinical specimens espe cially poly microbial samples (Table 1). Prevalence of nocardiosis in sputum (238 specimens), BAL (143 specimens) and cutaneous abscess (45 specimens) were 1.6%, 1.3%, 2.2%, respectively. In our study, we isolated one Nocardia spp. from cutaneous abscess of patient with Pemphigus disorder. Discussion In scientific resource, recommended the use of paraffin baiting technique for isolation Nocardia from polymicrobial flora such as sputum [18]. Nocardia spp. utilized of paraffin wax as the sole carbon source [15, 19]. It has been reported different medium containing antibiotic for isolation this organism such as chloramphenicol with sabouraud dextrose agar. Some species of the genus Nocardia are susceptible to chloramphenicol [16]. A wide range of nocardiosis occurs in Immunocompromised and immunosuppressive patients [8-10]. Mycobacterium tuberculosis mimicking pulmonary nocardiosis so isolation and identification Nocardia is very important because treatment in two organisms is difficult. In a study by Mishra and colleagues in 1969, they investigated 555 clinical specimens such as sputum, BAL and Gastric lavage and were positive respectively 10, 1 and 1 about Nocardia spp. [19]. Singh et al surveyed 1510 sputum specimens and results showed paraffin baiting method has higher efficacy of sabouraud dextrose agar [17]. Another study by Venugopal et al were examined 350 sputum, BAL, pleural fluid, pus , biopsy specimens and isolated 15 strains of Nocardia [20]. A study in 2001 from Iran, Eshraghi et al surveyed 142 sputum specimens and was positive 1 isolate (0.7%) but in our study was positive 4 isolates (1.3%) of 291 sputum of patients with suspected tuberculosis. The results show that Nocardia infection is increasing in Iranian patients. The reports showed paraffin baiting technique is more selective and effective than usual medium and paraffin agar. Conclusions We recommended that the be used of paraffin baiting technique for Nocardia isolation in clinical laboratories. due to aging and autoimmune or immune disorders in Iranian patients, isolation Nocardia spp is very necessary for treatment. Acknowledgments This study was supported by Tehran University of Medical Sciences, Deputy of Research. References 1.Eshraghi SS Molecular typing of Nocardia species. J Med Bacteriol 2012; 1(1): 38-45. 2.Budzik JM, Hosseini M, Mackinnon AC Jr, et al. Disseminated Nocardia farcinica: literature review and fatal outcome in an immunocompetent patient. Surg. Infect 2012; 13(3): 163-170. 3.Hollick GE. Nocardiosis. clinical microbiology newsletter 1988; 10(14): 105-109. 4.Eshraghi S, Amin M. Nocardia asteroides complex in patient with symptomatic pulmonary nocardiosis in a patient with bronchiectasis. Iran J Public Health 2001(3-4); 30: 99-102. 5.Stevens DA, Pier AC, Beaman BL, et al. Laboratory evaluation of an outbreak of nocardiosis in immunocompromised hosts. Am J Med 1981; 71(6): 928-934. 6.Brown-Elliott BA, Brown JM, Conville PS, et al. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin Microbiol Rev 2006; 19(2): 259-282. 7.Patel MP, Kute VB, Gumber MR, , et al. Successful treatment of Nocardia pneumonia with cytomegalovirus retinitis coinfection in a renal transplant recipient. Int Urol Nephrol 2012; 45: 581-5. 8.Poonwan N, Kusum M, Mikami Y, , et al. Pathogenic Nocardia isolated from clinical specimens including those of AIDS patients in Thailand. Eur J Epidemiol 1995; 11(5): 507-512. 9.Srifuengfung S, Poonwan N, Tribuddharat C, et al. Prevalence of Nocardia species isolated from patients with respiratory tract infections at siriraj hospital, Thailand. J Infect Dis Antimicrob Agents 2007; 24: 1-6. 10.Sahathevan M, Harvey FA, Forbes G, et al. Epidemiology, bacteriology and control of an outbreak of Nocardia asteroides infection on a liver unit. J Hosp 1991; 18: 473-480. 11.Das D. Actinomycosis in fine needle aspiration cytology. Cytopathology 1994;5(4): 243-250. 12.Wada R., Itabashi C, Nakayama Y, et al. Chronic granulomatous pleuritis caused by Nocardia: PCR based diagnosis by nocardial 16S rDNA in pathological specimens. J Clin Pathol 2003; 56(12): 966-969. 13.Gupta N, Srinivasan R, Kumar R, et al. Two cases of nocardiosis diagnosed by fineà ¢Ã¢â€š ¬Ã‚ needle aspiration cytology: Role of special stains. Diagn Cytopathol 2011; 39(5): 363-364. 14.Shawar RM, Moore DG, LaRocco MT. Cultivation of Nocardia spp. on chemically defined media for selective recovery of isolates from clinical specimens. J Clin Microbio l 1990; 28(3): 508-512. 15.Narang P, Dey S, Mendiratta D. Paraffin slide culture technique for Baiting Non-Tuberculous Mycobacteria. Indian. J. Tuberc 2000; 47(4): 219-222. 16.Garrett M, Holmes H, Nolte F. Selective buffered charcoal-yeast extract medium for isolation of Nocardiae from mixed cultures. J Clin Microbiol 1992; 30(7): 1891-1892. 17.Singh M, Sandhu RS, Randhawa HS. Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum. J Clin Microbiol 1987; 25(1): 176-177. 18.  Yu C T, Chua JA. Nocardiosis. PJMID 2001; 30: 56-61. 19.Mishra S, Randhawa H. Application of paraffin bait technique to the isolation of Nocardia asteroides from clinical specimens. Appl Microbiol 1969; 18(4): 686-687. 20.Venugopal PV, Taralakshmi VV, Subramanian S, , et al. Nocardia species from bronchopulmonary infections and mycetomas. Sabouraudia 1980; 18(1): 11-18. Figure 1. Isolation and growth Nocardia on paraffin coated glass rod Figure 2. Nocardia grown on nutrient agar medium 1

Double Cross

The smoke rising like steam from the open manhole, gave a curtain to a dark figure dressed in black, long cloak floating at the back .His presence scared away the crowding street cats lurking and prowling in the dark alley dustbins. The fall of the dustbin lid echoed through the street like a symbol in an orchestra. Cars seldom passed this area of town, notorious for crime. His destination, a small entrance bordered by neon lights which echoed through out the mist. As he neared the door a small spy hole slid open. The man spoke his words, muffled through his black scarf. After several seconds the doors locks slid open and the door followed. The glowing mist sucked into the open door. A man tower approached the light, built as wide as he was tall. Final checks were made on the man and he was allowed to enter. The radio was on. The time read quarter to ten. A man confessing his hard life working in an office for a boss he hates to the number one radio station in the area. These one-to-one chats with the air host keep the lonely people entertained at night. Detective Ventura turned it off and sighed. He sat peering into the dark alley where our mysterious character had disappeared moments before. The car door opened. A small man with mousy brown hair round chubby cheeks entered the car. His appearance mimicked his attitude. It was his first day. An uneasy silence surrounded the car, rain started to fall slowly and steadily onto the windscreen. It got heavier with time and the silence grew. The repetitive motion of the wipers swishing back and forth was causing Malcolm to sweat. Ventura noticed Malcolm's agitated motion. He decided he had to break the ice. â€Å"So how'd u feel first day out on the job. It's a hell of a lot different from the training aint it?† Ventura said breaking the silence. â€Å"Oh yeah, It's really exciting for me†¦Ã¢â‚¬ ¦what you think our next move is sir?† stuttered Malcolm. â€Å"Well son we gotta sit tight for a while. We don't want go bustin in there when we aint got no leads† he said proudly feeling wise and experienced. Their conversation was interrupted by a gunshot. The room was small but had many doors. He offered to take his coat, but he refused sternly. A look of suspicion was exchanged between the two unlikely gentlemen, when the moment was torn by a voice so strong and deep that it demanded respect. â€Å"Who the hell is it Mike? Are you gonna show um through or do I have to get off my death bed to meet him.† This voice came from a small silver haired man with definite Italian roots. His skin slightly faded from tan to grey. The man lay upon his bed propped up by a mountain of pillows. How a voice of such power and strength could come from this man was hard to believe. As he entered the room the old man glanced across. Mike announced, â€Å"Mr Leony, the man you requested is here with a proposition for you.† His stern voice was irritating â€Å"Ah great I have been expecting you. I feel that your skills may be of some use. Before I give you any details I want you to answer me a few questions. Firstly what's your name?† â€Å"My name†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦..Andreas† hesitating slightly. Mr Leony looks him up and down, then smiles, â€Å"Good can I call you Andy?† â€Å"I'd prefer if we used no names during any of our meetings.† He removed his long black coat. The thud as it hit the ground raised eyebrows. â€Å"Mike I thought you checked him?† â€Å"I did boss, he was clean†¦..at least I think he was!† â€Å"That's another thing you need to improve security around here!† said Andreas, as he pulled from his coat a revolver. Eyes widen. The room is silenced. A shot fired. Mike hit the floor. â€Å"What are you doing!? Stop I beg of you there's no need for this. Who sent you!?† pleaded Antonio Leony. Andreas was considering whether or not to tell Mr Leony who sent him. He looked at his watch. It read five minutes to ten. Ventura bounced from his seat. The coffee flew onto the windscreen. They both looked at each other and simultaneously opened their doors. Time stood still at six minutes to ten as both officers rushed to the neon glare. They both backed up against the wall, guns at the ready. The gel from Malcolm's hair was running into his eyes. He became agitated again, â€Å"Sir what shall†¦.† â€Å"Shhhh!† Ventura interrupted. They both stood silent waiting for a sound inside or movement. Something to give them an insight. Andreas spoke. â€Å"I'm very sorry old man† he said reluctantly. â€Å"At least do me the honour of knowing which back stabbing bastard set me up? Who was it?† he said bravely. â€Å"What good is it going to do you?†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦but I suppose it's not going to make much difference whether or not I tell you. Ok it was John Maverick.† â€Å"What that Bastard!† he was raging like a bull. â€Å"I find it hard to end such a man but, you win some, you lose some.† He spoke respectfully, the gun fired. The second gunshot went off piercing Ventura's ears. They had to make a move; they burst in. The warm air hit them. They came to the door. It opened with a slight creak. Ventura made sure the surroundings were clear and checked the body for life signs. There were none. The curtains flailed in the wind. Our mysterious character had disappeared. Venture heard the fire escape ladder slide. His eyes met Malcolm's and they synchronically edged toward the window. Unveiling the curtain they watched as the dark figure entered a black saloon car and drove away leaving the spray from the wet road in its wake. He had escaped. Ventura and Malcolm got back to the station. Their captain shouted to them. They went to his office. The room was small with thin walls. Ventura had often been battered verbally by the captain. There was a name plate, which read Captain John Maverick. Expecting a good telling off, the two officer's eyes were firmly fixed on the ground. â€Å"Ah hell guys, what happened out there?†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Look more to the point we got an anonymous phone call tipping us off about our mystery man that I have had you boys tail for the last 2 weeks† the captain spoke casually which came as a shock to the officers, Ventura spoke anxiously â€Å"What did it say sir?† â€Å"Well it gave us a meeting point and a time. It's six tomorrow morning at Swallow Lake. You feeling up to it Ventura?† â€Å"Hell yeah sir!† Ventura pounced. â€Å"And take the rookie with you. This is good experience for him.† Captain said smiling. A bird flew by. Its frozen frame glided across the lake. It dipped slowly out of sight. A moment later it burst into view fluttering its wings. It soared into the sky and began to circle the lake like a vulture would its prey†¦.As Ventura followed the bird he noticed the sky; it was red. He remembered the old saying his grandfather used â€Å"Red sky at night Shepherd's delight, Red sky in the morning Shepherd's warning†, he whispered. The moment was broken by a black saloon pulling up. A man dressed in black got of out the car and slammed the door. More birds burst into the sky and began to circle. Ventura saw a tall black man approach the pier. He reached for his binoculars. â€Å"Wait! It can't be.† His eyes widened as he confirmed his fears. â€Å"Who is it sir? Who is it?† Malcolm inquired. A knock came at the car window. Both heads swivelled. A tall man held a gun pointed at Malcolm. â€Å"Get out the car, and no funny stuff. I got a loaded gun here!† he said having the upper hand. They were taken to a lodge. Inside their fears were confirmed. Their captain had set them up. â€Å"Hi guys†¦Ã¢â‚¬ ¦.well what can I say, Ventura if you had just messed up like you did every other time you would be sitting here right now. You got to close damn it!† he said as if to give them some sort of consolation. The Captain called to the man, â€Å"Andreas have you collected the money?† he said anxiously â€Å"Yes but I'm afraid I can't let you have it. You see I'm a solo man I work for me and me only† he said smugly. Meanwhile Ventura had slipped his penknife from his pocket and was cutting away at the ropes which bound him and Malcolm to the chair, â€Å"What the hell you talking bout? We had a deal. If you go back. I'll kill you† he said furiously, before he could reach for his gun Andreas shot him in the chest. The captain fell to the ground. Andreas turned to the two men tied to the chairs back to back. â€Å"Sorry fellas, honestly I was real impressed with you two being able to track me down. That aint an easy job, but you have got to die!† Andreas poured petrol in a circle around the men and doused the walls and floor. The room spun faster, and faster, and faster, but Ventura kept his cool. Time was running out, Andreas lit his cigar, â€Å"It's been a pleasure gentleman.† He went to drop his match. Ventura rose, Andreas flinched and stumbles back. Ventura reached for his second gun in his sock. Andreas pulls back his coat and grasped his gun. Ventura fired. Andreas fired. Time stopped. The exchange of fire made Malcolm faint. Both men lay motionless on the floor. Ventura lay still as ice. Blood mixed in with the petrol and ran down the cracks in the floor. Malcolm came around and saw the two men lying on the floor beside him. He calls to Ventura, â€Å"Sir!, come on get up. Sir?† he shouted. Ventura lay†¦dead? Malcolm's attention was turned to a rising Andreas. The man rose, grasping his side where he had been shot. He leant against the door frame, his back to Malcolm. Andreas turned and reaching for his gun aims it at Malcolm. Malcolm closes his eyes and prays to god. Three gun shots are fired, but Malcolm felt nothing. When he finally opens his eyes he's back in the same room with Andreas lying dead on the floor in front of him. He hears the faint cough of Ventura and turned quickly to find him nursing a shot wound to his stomach and still pointing a smoking gun in Andreas's direction. Ventura got back to his feet and ventured over to his Captain. he looked down at a man for whom he now felt nothing but sorrow for him. The captain's eyes, as if mesmerised, gazed at the roof. They drifted on to Ventura's eyes. Ventura drops and holds the captain by his back. The captain spoke with a groan. â€Å"Ah, hell. Things just didn't turn out well. I'm sorry Ventura. This wasn't anything personal. He coughed and he died. Ventura eyes glazed like ice. The moment was broken by the sound of a dozen police cars racing to the scene. The birds broke from their circle and descended. The sky was turned from red to blue by the rising sun.

Logic

Is communication e cattle without the logical connectives such as or, ND, implies, not, if and only if ? [200 words] 5. Why we treat mathematical truths as certain? Why in logic we me- aphasia on autologies† 2 rather than contradictions? [200 words] (Consistency) an attribute of a logical system that Is so constituted that none of the propositions deducible from the axioms contradict one another 4. [n] – a harmonious uniformity or agreement among things or parts Tautology: a statement that is necessarily true (like 2+2-4) 1 6. What is wrong with the following argument?The more you study, the more you learn. The more you learn, the more you know. The more you know, the more you forget. The more you forget, the less you know. The less you know, the less you learn. So, why study? 7. Analyze the following argument: God is All Powerful, omnipotent and omniscient. If God can do anything, can He create something that even He can't a etc? (e: A stone so heavy that He can't lift It) God can do anything that Is Possible. Even God can't do something that Is not possible to be done Can God create a stone so heavy that even He can not lift it? 8.In a harsh totalitarian country an innocent person is arrested on Sunday evening and Marilyn condemned to execution, which they are told will take place on one of the following eve mornings. To make matters worse, they are told that they will not know the day before which morning it will be. After several hours torment the prisoner fails into a peaceful sleep as they realize that such a threat cannot be carried out. They reasoned thus: The execution cannot take place on Friday morning; for if they are still alive on Thursday night then the execution must take place on Friday.But they were told that they would not know the day before which day it would be. So it cannot be Friday, and so Friday can be counted out as a possibility. But by the same reasoning it cannot be Thursday either. For if they are still alive on Wedn esday night then the execution must take place on Thursday. But they were told that they would not know the day before which day it would be. So it cannot be Thursday, and so Thursday can be counted out as a possibility. But by the same reasoning it cannot be Wednesday either†¦Ã¢â‚¬ ¦. The same reasoning covers Wednesday, Tuesday and Monday and so the prisoner can have a sound nights sleep.The prisoner is, however, greatly surprised to ND themselves facing the executioner on Wednesday (or indeed any other) morning. What went wrong with the prisoner's reasoning? Write down the steps clearly and show how paradox 3 arises in the above situation? Paradox: An apparently sound argument leading to a contradiction. Some famous 2 PHI 142: Introduction to Logic Mid Semester Exam Date: 19 February 2013 Department of Humanities and Social Sciences Indian Institute of Technology Kanata Answer all of the following questions. Write answers in a separate booklet.Max. Marks: 30 Time: 2 hours; 1 7. 30-19. 30 PM We may define faith as the firm belief in something for which there is no evidence. Where there is evidence, no one speaks of . We do not speak of faith that two and two are four or that the earth is round. We only speak of faith when we wish to substitute emotion for evidence. †¦Ã¢â‚¬ ¦ Bertrand Russell Answer whether the following statements are True or False. Justify it prefer- ably with a very short answer. 1. The following argument is valid: If computers can reason, they can ask questions. Computers can reason.Therefore, computers can ask questions . The following argument is an instance of Fallacy of accident fallacy . † Oh my lord, don't take any courses in the biology department not even as open elective, as they are really tough and terrible. I took EBBS:322 and it was a disaster. † 3. The categorical propositions, All who are non intelligent are non-college students and all who are college students are intelligent are contradictory to each other 4. I am sure that you will agree that we should not vote Mr.. Panky Saxons for the next presidential elections in this hostel.If he gets elected, we will all be deprived of mom of the privileges that we are enjoying at this moment. Anyone not agreeing with this will be fired immediately from this wing A-top. In the above argument, the arguer is said to be committing straw-man fallacy. 5. False Cause fallacy is committed when the conclusion rests on the presumption of a causal link between two phenomena, when in fact all that has been shown is a correlation. No concepts are expressive intuitions on aversion converts to All concepts are non- expressive intuitions 7.The categorical propositions, All logic students are bright people No logic students are non-bright people re contradictory to each other. 8. The following argument is invalid If Newton's theory is correct, then light should not be deflected by gravity. In this experiment, we can see that light is deflected by gravity . Therefore, Newton's theory is not correct PHI 142: Introduction to Logic Mid Semester Exam Date: 19 February 2013 2. Which of the following passages contains arguments and which are not I. E. , Non-arguments 1 . If there is one theme that will be the topic of digital business this year, it is mobile.Commerce, which tracks Web and mobile usage, published a report about what hap- need in 2012, and what to expect in 2013. It shows that the effects of a movement toward mobile are everywhere, from shopping to media to search. According to the report, â€Å"2013 could spell a very rocky economic transition,† and businesses will have to scramble to stay ahead of consumers changing behavior (anytime. Com). 2. Ordinary glass is soda lime glass and is a mixture of silicates of sodium, potassium, calcium and aluminum etc. It is brittle because its molecular structure is composed of tetrahedral crystals.These crystals do not have a good large area orderly resealing structure. 3. People are not free, although human freedom has been highly prized throughout our history. Its value is affirmed by religious doctrines. Civil and criminal Justice systems incorporate the idea that behavior is freely chosen into sentencing guidelines. Clearly it has been an important concept in many areas of human activity. 3. Identify the type of Fallacy of Weak Induction from the following arguments 1 . I cracked most of my crucial exams (board exams) in the past while wearing my green tea shirt and writing an exam with my parker pen.Therefore, green color tea hire with a parker pen is a lucky combination and brought lot of success. Hence I should not forget to take it when I go for an exam again for achieving success in the exam. 2. If once a man indulges himself in murder, very soon he comes to think little of robbing; and from robbing he comes next to drinking and Sabbath breaking, and from that to incivility and procrastination. Once begin upon this downward path, you never know whe re you are to stop. Many a man has dated his ruin from some murder or other that perhaps he thought little of at the time. 3.Famous theologian William Palely argument from design suggests that a watch and the universe are similar as both display order and complexity. If order and complexity constitute evidence of design in the case of a watch, though, then they must also constitute evidence of the product of intelligent design that the universe must be a product of intelligent design too. 4. In 1742, Christian Goldberg conjectured that every even number greater than 2 is the sum of two primes. Mathematicians have been trying to prove Schoolbag's conjecture ever since, but no one has succeeded in doing so.After two and a half entries, I think we can safely conclude that Goldberg was wrong. 4. Identify the type of Fallacies of Relevance 4 committed by the following arguments. Justify your answer with a brief explanation 1 . Social Security old age benefits ensure you can retire with d ignity. If you become injured or are unable to work, Social Security disability benefits will help keep you afloat. But, Mrs.. Sheila's arguments against cuts in Social Security are worthless. As a recipient of Social Security benefits, she would naturally be expected to argue exactly the way she does. Page 2 of 4

Knights of Labor Essay - 731 Words

to be or not to be, that is the question. When one meets Gould for the first time, one witnesses a tremendous power: no one can question his charisma and compelling personality. But under the facade, behind the mask of great glory and munificence lies the true Gould, the Gould who desires to enslave the working man, to nullify his basic rights, and crush every bit of resistance against injustice. The duplicity of the magnate is not always obvious, but it is evident in his dealings with the Knights of Labor. speak softly but carry a big stick, and you will go far. No other union had come into such heated conflict with Gould as did the Knights, as no other union had been strong enough to challenge Gould himself. But in the end, even the†¦show more content†¦Hoxie, believing that the strike was a direct violation of the agreement reached between Gould’s Missouri Railroad Company and the Knights during the Wabash strike not to strike before arbitration was attempted on any issue, began a violent campaign against the Knights of Labor, firing its members and recruiting new workers. The Knights of Labor were eventually forced into a desperate state. In fact, the later strike was not instigated by Powderly, but by one of his officers Martin Irons, who chose to defy the authority of Powderly when a man named Hall was discharged by the Texas and Pacific Railroad. The strike, an indirect effect of a mass influx of uninformed newcomers to the union, marked the beginning of unorganized struggle of the union and the end of Powderly’s influence. The outcome of the strike was so disastrous for the labor union that it caused even the United States government to fear Gould’s autocratic power; the president himself had urged Gould not to stand in the way of arbitration. In a later encounter between Powderly and Gould, Gould—seeming a mediatory man— appears to yield to Powderly’s demands by agreeing to order Hoxie to readmit workers regardless of union affiliation (but he had in fact written the telegram so that it implied that Hoxie continue with his policies). When the newspapers leaked out the information, Gould responded by informing Powderly that he had no power toShow MoreRelatedKnights of Labor Essay1246 Words   |  5 Pages The Knights of Labor represented the pinnacle of the up lift labor movement. They, at one time, had membership that numbered in the hundreds of thousands and nearly hit a million members. This organization was unique in its time because it espoused many of the ideals we hold today as statutory for an ethical and equitable society as well as employee employer relationship. The Knights of Labor did not begrudge industry or the capitalism, more over they were less of a concern than the organizations Read More The Knights of Labor Essay1327 Words   |  6 PagesThe Knights of Labor represented the pinnacle of the up lift labor movement. They, at one time, had membership that numbered in the hundreds of thousands and nearly hit a million members. This organization was unique in its time because it espoused many of the ideals we hold today as statutory for an ethical and equitable society as well as employee and employer relationships. The Knights of Labor did not begrudge industry or capitalism, moreover they were less of a concern than the organization’sRead MoreA Brief Look at Terrence Vincent Powderly1461 Words   |  6 Pagessecretive organization named the Knights of Labor. 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